{"id":52591,"date":"2026-10-13T12:05:57","date_gmt":"2026-10-13T09:05:57","guid":{"rendered":"https:\/\/umfst.ro\/?p=52591"},"modified":"2026-10-13T12:05:59","modified_gmt":"2026-10-13T09:05:59","slug":"workshop-sistem-de-secventiere-oxford-nanopore-promethion-2-integrated","status":"publish","type":"post","link":"https:\/\/umfst.ro\/hu\/blog\/workshop-sistem-de-secventiere-oxford-nanopore-promethion-2-integrated\/","title":{"rendered":"Workshop \u2013 Sistem de secven\u021biere Oxford Nanopore PromethION 2 Integrated"},"content":{"rendered":"<p class=\"wp-block-paragraph\">\u00cen cadrul proiectului FOCUS: Formare \u0218i Orientare pentru Cercet\u0103torii UMFST \u00een S\u0103n\u0103tate, v\u0103 invit\u0103m s\u0103 participa\u021bi la un workshop de instruire practic\u0103 dedicat unei tehnologii emergente \u00een cercetarea biomedical\u0103: Oxford Nanopore PromethION 2 Integrated \u2013 sistem de secven\u021biere de nou\u0103 genera\u021bie cu citiri lungi<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Loca\u021bie: Centrul Avansat de Cercet\u0103ri Medicale \u0219i Farmaceutice (CCAMF) \u2013 Laborator BIOMOL<br>Ora: 9:00 -18:00<br>Grup \u021bint\u0103: doctoranzi UMFST, postdoctoranzi UMFST<br>Data: 14.10.2026 \u2013 16.10.2026<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Workshopul \u00ee\u0219i propune familiarizarea participan\u021bilor cu principiile secven\u021bierii prin nanopori \u0219i cu utilizarea practic\u0103 a sistemului PromethION 2 Integrated pentru secven\u021bierea direct\u0103, \u00een timp real, a moleculelor de ADN \u0219i ARN, cu citiri lungi \u0219i foarte lungi, \u00een cercetarea biomedical\u0103 \u0219i \u00een diagnosticul molecular.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Workshopul \u00ee\u0219i propune familiarizarea participan\u021bilor cu principiile secven\u021bierii prin nanopori \u0219i cu utilizarea practic\u0103 a sistemului PromethION 2 Integrated pentru secven\u021bierea direct\u0103, \u00een timp real, a moleculelor de ADN \u0219i ARN, cu citiri lungi \u0219i foarte lungi, \u00een cercetarea biomedical\u0103 \u0219i \u00een diagnosticul molecular. Prin demonstra\u021bie \u0219i discu\u021bii aplicate, workshopul urm\u0103re\u0219te familiarizarea participan\u021bilor cu o tehnologie emergent\u0103 de secven\u021biere \u0219i sprijin\u0103 conversia rezultatelor \u0219tiin\u021bifice \u00een aplica\u021bii cu relevan\u021b\u0103 clinic\u0103.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Tematica abordat\u0103<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Principiile secven\u021bierii prin nanopori proteici: detec\u021bia la nivel de molecul\u0103 unic\u0103 \u0219i citirea direct\u0103 a ADN\/ARN<\/li>\n\n\n\n<li>Preg\u0103tirea probelor:\n<ul class=\"wp-block-list\">\n<li>Controlul calit\u0103\u021bii \u0219i cuantificarea acizilor nucleici<\/li>\n\n\n\n<li>Prepararea libr\u0103riilor (ligare, barcoding \u0219i multiplexare)<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>Operarea sistemului P2i:\n<ul class=\"wp-block-list\">\n<li>\u00cenc\u0103rcarea \u0219i verificarea celulelor de reac\u021bie (flow cells)<\/li>\n\n\n\n<li>Configurarea \u0219i monitorizarea rul\u0103rilor \u00een software-ul MinKNOW<\/li>\n\n\n\n<li>Basecalling \u00een timp real pe unitatea de calcul integrat\u0103<\/li>\n\n\n\n<li>Sp\u0103larea \u0219i reutilizarea celulelor de reac\u021bie<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>Gestionarea datelor de secven\u021biere: formatele POD5, FASTQ \u0219i BAM<\/li>\n\n\n\n<li>Aplica\u021bii: secven\u021biere de genom \u00eentreg, variante structurale, metilare ADN, secven\u021biere direct\u0103 de ARN, metagenomic\u0103 \u0219i diagnostic microbiologic rapid<\/li>\n\n\n\n<li>Prezentarea platformei portabile complementare MinION Mk1D<\/li>\n\n\n\n<li>Testarea \u00a0fluxului de lucru, de la \u00eenc\u0103rcarea probei la vizualizarea datelor \u00een timp real<\/li>\n\n\n\n<li>Discu\u021bie: integrarea secven\u021bierii cu citiri lungi \u00een proiectele doctorale \u0219i traseul de la rezultatul \u0219tiin\u021bific la aplica\u021bia cu relevan\u021b\u0103 clinic\u0103<\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p>\u00cen cadrul proiectului FOCUS: Formare \u0218i Orientare pentru Cercet\u0103torii UMFST \u00een S\u0103n\u0103tate, v\u0103 invit\u0103m s\u0103 participa\u021bi la un workshop de instruire practic\u0103 dedicat unei tehnologii emergente \u00een cercetarea biomedical\u0103: Oxford Nanopore PromethION 2 Integrated \u2013 sistem de secven\u021biere de nou\u0103 genera\u021bie cu citiri lungi Loca\u021bie: Centrul Avansat de Cercet\u0103ri Medicale \u0219i Farmaceutice (CCAMF) \u2013 Laborator [&hellip;]<\/p>\n","protected":false},"author":25,"featured_media":52592,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_relevanssi_hide_post":"","_relevanssi_hide_content":"","_relevanssi_pin_for_all":"","_relevanssi_pin_keywords":"","_relevanssi_unpin_keywords":"","_relevanssi_related_keywords":"","_relevanssi_related_include_ids":"","_relevanssi_related_exclude_ids":"","_relevanssi_related_no_append":"","_relevanssi_related_not_related":"","_relevanssi_related_posts":"17398,25500,47165,50210,47811,44941","_relevanssi_noindex_reason":"","footnotes":""},"categories":[38],"tags":[],"subiect":[208],"class_list":["post-52591","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articol","subiect-info-umfst"],"meta_box":{"text_b6uo101re2g":"","corp_citat":"","autor_citat":"","imagine_autor_citat":false,"afiliere_autor_citat":""},"_links":{"self":[{"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/posts\/52591","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/users\/25"}],"replies":[{"embeddable":true,"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/comments?post=52591"}],"version-history":[{"count":1,"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/posts\/52591\/revisions"}],"predecessor-version":[{"id":52593,"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/posts\/52591\/revisions\/52593"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/media\/52592"}],"wp:attachment":[{"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/media?parent=52591"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/categories?post=52591"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/tags?post=52591"},{"taxonomy":"subiect","embeddable":true,"href":"https:\/\/umfst.ro\/hu\/wp-json\/wp\/v2\/subiect?post=52591"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}